BeNa Culture Collection
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| Culture medium | BNCC363892 |
| Description | Modified PYG Broth |
| Composition | Pancreatic Digest of Casein:5.0g, Peptone:5.0g, Yeast Extract:10.0g, Beef Extract:5.0g, Glucose:5.0g, K2HPO4:2.0g, Tween 80:1.0ml, CaCl2.2H2O:0.01g, MgSO4.7H2O:0.02g, K2HPO4:0.04g, KH2PO4:0.04g, NaHCO3:0.4g, NaCl:0.08g, Resazurin:1.0mg, Hemin:5mg, Vitamin K1:1mg, C3H7NO2S.HCl:0.5g, pH:7.2±0.2(25℃) |
| Growth conditions | 37 ℃; 3-5 days; anaerobic |
| Subculture procedure | ① Prepare one liquid culture medium with a diameter of 18mm, approximately 10mL (placed in an anaerobic environment for 24 hours of deoxygenation in advance); ② Dissolve the Frozen vials in a 37 ℃ water bath and shake rapidly for dissolution; ③ Suck the bacterial solution back into the test tube liquid culture medium; ④ Place the liquid test tube under the above cultivation conditions for cultivation. If the bacterial solution is turbid or there is a large amount of bacterial growth at the bottom, it can be used. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | G(+), Spherical shape |
| Sharing mode | Public welfare sharing |
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