BeNa Culture Collection
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| Culture medium | BNCC376181 |
| Description | Modified YCFA Medium Base |
| Composition | Trypticase:10.0g, Yeast extract:2.5g, Glucose:5g, K2HPO4:0.45g, KH2PO4:0.45g, NaCl:0.9g, MgSO4.7H2O:45.00mg, CaCl2.2H2O:90.00mg, Resazurin:1.0mg, Volatile fatty acids:10ml, NaHCO3:4.0g, Cysteine hydrochloride(C3H7NO2S.HCl):1.0g, Hemin(C34H32ClFeN4O4):10.0mg, Vitamin solution:10ml |
| Growth conditions | 37 ℃; 4-5 days; Strictly anaerobic; |
| Subculture procedure | ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | Liquid medium turbid, G+(blue purple), streptococcus |
| Sharing mode | Public welfare sharing |
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