Long chain Dole bacteria|384596 |BNCC

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Dorea longicatena

Culture medium	BNCC376181
Description	Modified YCFA Medium Base
Composition	Trypticase:10.0g, Yeast extract:2.5g, Glucose:5g, K₂HPO₄:0.45g, KH₂PO₄:0.45g, NaCl:0.9g, MgSO₄.7H₂O:45.00mg, CaCl₂.2H₂O:90.00mg, Resazurin:1.0mg, Volatile fatty acids:10ml, NaHCO₃:4.0g, Cysteine hydrochloride(C₃H₇NO₂S.HCl):1.0g, Hemin(C₃₄H₃₂ClFeN₄O₄):10.0mg, Vitamin solution:10ml
Growth conditions	37 ℃; 4-5 days; Strictly anaerobic;
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions	-80 ℃
Safety level	1
morphology	Liquid medium turbid, G+(blue purple), streptococcus
Sharing mode	Public welfare sharing