BeNa Culture Collection
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| Growth conditions | Medium 1203, anaerobic (88% air, 7% H₂, 5% CO₂), 37°C Incubation time: 1-2 days |
| Subculture procedure | ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow. |
| Storage conditions | Cryovial Tube |
| Safety level | 1 |
| morphology | pure |
| Sharing mode | Public welfare sharing |
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