BeNa Culture Collection
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| Culture medium | BNCC373765 |
| Description | LB Medium with 10μg/mL Chloramphenicol |
| Composition | Yeast extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, Chloramphenicol:10µg/mL, pH:7.0±0.2(25℃) |
| Growth conditions | 37 ℃; 18-24h; aerobic |
| Subculture procedure | ① Prepare 1-2 of the above agar plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colonies are pure, 2-4 mm, circular, convex, opaque, pale yellow colonies with irregular margins and a smooth, glistening, moist texture that is easily picked up, composed of Gram-positive (blue-violet) cocci. |
| Sharing mode | Public welfare sharing |
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