BeNa Culture Collection
Culture medium | Yeast extract: 3.0, malt extract: 3.0, glucose: 10.0, casein peptone: 5.0, agar: 20.0,pH:6.2±0.2(25 ℃) |
Subculture procedure | ① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried. |
Growth conditions | 30°C;24-48h; aerobic |
Storage conditions | -80 ℃ |
Safety level | 1 |
morphology | The colony diameter is 1-2mm, round, opaque, gray-white on the front, raised in the middle, smooth surface, moist texture, G (blue-purple), ovoid |
Sharing mode | Public welfare sharing |