Shigella-like nucleic acid detection kit (fluorescent PCR method)|BNCC373977 |BNCC

Essential Information Related Products

Plesiomonas shigelloides nucleic acid detection kit (Fluorescent PCR method)

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 15 μL, template 2-10 μL, and the rest supplemented with water; Experimental reaction conditions: the first step is 96 ℃ for 10min, the second step is 95 ℃ for 10s, the third step is 55 ℃ for 40s, and the second to third steps are cycled 40 times; Fluorescence Channel: FAM.
Growth conditions	37 ℃;18-24h; aerobic;
Storage conditions	-20 ℃; protected from light
Safety level	1
application	Suitable for nucleic acid detection of Shigella-like bacteria
Sharing mode	Public welfare sharing