Rhodopseudomonas palustris nucleic acid detection kit (fluorescent PCR method)|BNCC372173 |BNCC

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Rhodopseudomonas palustris nucleic acid detection kit (Fluorescence PCR method)

Culture medium	Ammonium chloride: 1.0, potassium dihydrogen phosphate: 0.5, magnesium chloride: 0.5, calcium chloride: 0.1, sodium chloride: 1.0, sodium acetate: 2.0, sodium succinate: 1.0, yeast extract powder: 0.5, peptone: 0.5, ferrous chloride: 1.8mg, cobalt chloride: 0.25mg, nickel chloride: 0.01mg, copper chloride: 0.01mg, manganese chloride: 0.07mg, zinc chloride: 0.1mg, boric acid: 0.5mg, sodium selenite: 0.01mg, sodium molybdate: sodium molybdate: 0.01g, biotin: 0.01mg, nicotinic acid: 0.035mg, calcium pantothenate: 0.01mg, thiamine hydrochloride: 0.03mg,VB12:0.005mg, pyridoxamine hydrochloride: 0.01mg, p-aminobenzoic acid: 0.02mg, agar: 13.0,pH:6.8(25 ℃)
Subculture procedure	Experimental preparation: refer to GB4789.10 or GB7918.5 to carry out the enrichment culture of the sample before the experiment; dissolve the various components in the kit on ice with the front, mix slightly and centrifuge for a short time, and then place them on ice for standby; Experimental reaction system (25 μL):qPCR mixed reaction solution 24 μL, template 1 μL; Experimental reaction conditions: the first step 95 ℃ 1min, the second step 95 ℃ 10s, the third step 51 ℃ 30s, the second to third steps cycle 40 times; Fluorescence channels: FAM, VIC.
Growth conditions	The culture temperature is 37 ℃; the culture time is 7-9 hours; the gas environment is protected from light and aerobic;
Storage conditions	-20 ℃, dark and refrigerated
Safety level	2
Separation substrate	Human lesion
application	Rapid detection of Rhodopseudomonas palustris
Sharing mode	Public welfare sharing