BeNa Culture Collection
Subculture procedure | Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 18 μL, template 2 μL, sterile ddH2O to 25 μL; Experimental reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 55 ℃ for 30s, and the second to third steps are cycled 40 times; Fluorescence Channel: FAM |
Storage conditions | -20 ℃ |
Safety level | 1 |
application | It can not be used for the diagnosis of clinical diseases by using fluorescence PCR technology to carry out specific in vitro amplification research and detection of goat pox virus DNA |
Sharing mode | Public welfare sharing |