Diarrheagenic Escherichia coli (DEC) nucleic acid detection kit (fluorescent PCR method)|BNCC361924 |BNCC

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Escherichia coli nucleic acid detection kit (QPCR)

Subculture procedure	1. Experimental preparation: dissolve the various components in the kit on ice before use, mix them slightly and centrifuge them briefly and place them on ice for later use; 2. Sample treatment: sample preparation shall be carried out in accordance with the "GB4789.6-2016 Diarrhea Escherichia coli Detection; 3. Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 23 μL, template 2 μL; 4. Reaction conditions: 95 ℃ 3min 1cycle;94 ℃ 10s, 55 ℃ 30s 40cycle; 5. Fluorescence channel: FAM;
Storage conditions	-20 ℃
Safety level	1
application	It is suitable for auxiliary diagnosis of diarrheogenic Escherichia coli (DEC) infection in water samples, stool samples, suspected contaminated water, food samples, etc.
Sharing mode	Public welfare sharing