Mycoplasma detection kit (fluorescence PCR method)|BNCC382141 |BNCC

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Mycoplasma nucleic acid detection kit (Fluorescent PCR method)

Subculture procedure	1. Experimental preparation: dissolve the components in the kit on ice, mix well, centrifuge for a short time and put them on ice for later use; 2. Sample processing: self-prepared or pre-extracted test sample template DNA; 3. Reagent preparation: reaction system (25 μL), take 15 μL of reaction solution, add 2 μL of template, 8 μL of DEPC-treated water, mix well, and centrifuge briefly; 4. Reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 58 ℃ for 30s, and the second to third steps are cycled 40 times. 5. Fluorescence channel: FAM;
Storage conditions	-20 ℃; protected from light
Safety level	1
application	Suitable for mycoplasma contamination detection in cell culture process
Sharing mode	Public welfare sharing