Human Staphylococcus nucleic acid detection kit (fluorescent PCR method)|BNCC372299 |BNCC

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Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	1. Detection experiment: dissolve the components in the kit on ice, mix well, centrifuge for a short time and put them on ice for later use; 2. Reagent preparation: reaction system (25 μL), take 15 μL of reaction solution, add 1 μL of template, 9 μL of DEPC-treated water, mix well, and centrifuge briefly; 3. Reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 61 ℃ for 30s, and the second to third steps are cycled 40 times. 4. Fluorescence channel: FAM;
Growth conditions	culture temperature 37 ℃; culture time 20 hours; gas environment aerobic;
Storage conditions	-20 ℃, dark and refrigerated
Safety level	2
application	For rapid detection of human staphylococcus
Sharing mode	Public welfare sharing