BeNa Culture Collection
Subculture procedure | Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 15 μL, template 2-10 μL, and the rest supplemented with water; Experimental reaction conditions: the first step is 50 ℃ for 2min; The second step is 95 ℃ for 30s; The third step is 5S at 95 ℃, 60 ℃ for 50s, and the third step is cycled 40 times; Fluorescence Channel: HEX. |
Storage conditions | -20 ℃; protected from light |
Safety level | 1 |
application | Suitable for nucleic acid detection of Salmonella typhimurium |
Sharing mode | Public welfare sharing |