BeNa Culture Collection
Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculture procedure | When using essential control products, they should be moved to room temperature (20~25 ℃) for 10 minutes, dissolved with 1mL sterile water or sterile physiological saline, with a minimum sampling volume of 200uL, a concentration value of 108 ~ 109PFU/mL, and a reference concentration of 8.8 × 108PFU/mL. |
Storage conditions | -20 ℃ |
Safety level | 1 |
application | The E. coli phage T7 quantitative standard quality control, by culturing E. coli host bacteria and phage T7, phage collection. The titer and lysis efficiency of the collected phage were determined by double-layer agar counting method, which was mainly used for the quality control of phage T7 positive. |
Sharing mode | Public welfare sharing |