BeNa Culture Collection
Subculture procedure | Glycerol + NB(1:1) is sterilized at 121 ℃ for 15min, the mixed solution is fully mixed with Escherichia coli phage T1 stock solution 1:1, injected into 1.5mLEP tubes, each tube is injected into 0.5mL, and stored in a refrigerator at -80 ℃. |
Safety level | 1 |
application | This E. coli phage T1 quantitative standard quality control is collected by culturing E. coli host bacteria and phage T1. The titer and lysis efficiency of the collected phage were determined by double-layer agar counting method, which was mainly used for the quality control of phage PhiX174 positive. |
Sharing mode | Public welfare sharing |