Bacillus cereus quantitative strain|BNCC370107 |BNCC

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Quantitative strains of bacillus cereus

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃, aerobic
Growth characteristics	The strain was cultured on nutrient meat juice agar medium at 37 ℃ for 3 days, and the thallus was rod-shaped, 0.61 μm × 1.22~2.13 μm, gram-negative, and did not produce spores.
Storage conditions	-20 ℃
Safety level	2
morphology	Light yellow, raised, moist, bright, G-bacillus
Separation substrate	Blood culture
application	determination of chlortetracycline content determination of chlortetracycline content determination of norchlortetracycline content determination of doxycycline content determination of oxytetracycline content determination of oxytetracycline content determination of tetracycline content determination of medium detection of quality control strain detection of food detection of BBL product quality control strain
Sharing mode	Public welfare sharing