BeNa Culture Collection
Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculture procedure | ① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried; |
Growth conditions | 30 ℃;18-24h; aerobic; |
Storage conditions | -80 ℃ |
Safety level | 1 |
morphology | The colony diameter is 1-2mm, round, neat edges, opaque, white on the front, light color, raised in the middle, smooth surface, bright surface, moist texture, easy to provoke, G-(red), bacilli |
Sharing mode | Public welfare sharing |