Green fluorescent hemorrhagic Escherichia coli O157:H7|BNCC363779 |BNCC

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Escherichia coli EHEC O157:H7-sfgfp green fluorescent

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	When using essential control products, they should be moved to room temperature (20~25 ℃) for 10 minutes, dissolved with 1mL sterile water or sterile physiological saline, with a minimum sampling volume of 200uL, and counted with 100 μg/mL canamycin LB plate, with a concentration value of 107 ~ 108CFU/mL and a reference concentration of 2.4 × 107CFU/mL.
Growth conditions	37 ℃;18-24h; aerobic
Storage conditions	-20 ℃
Safety level	2
application	sfgfp, also known as enhanced green fluorescent protein, can emit green fluorescence under blue light excitation, with stable chemical properties, almost no toxicity to cells, and easy to detect. The green fluorescence characteristics of sfgfp were introduced into the hemorrhagic Escherichia coli O157:H7 as a tracer bacteria, and its fluorescence properties could be used to detect the hemorrhagic Escherichia coli O157:H7.
Sharing mode	Public welfare sharing