Clostridium butyricum nucleic acid detection kit (fluorescent PCR method)|BNCC372478 |BNCC

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Clostridium butyricum nucleic acid detection kit (Fluorescence PCR method)

Culture medium	Yeast extract: 3.0, beef extract: 10.0, glucose: 5.0, tryptone: 10.0, sodium chloride: 5.0, soluble starch: 1.0,L-cysteine hydrochloride: 0.5, sodium acetate: 3.0, agar: 12.5,pH:6.8±0.2(25 ℃)
Subculture procedure	1. Experimental preparation: dissolve the components in the kit on ice, mix well, centrifuge for a short time and put them on ice for later use; 2. Sample processing: After the positive quality control is dissolved, it is then placed on ice for later use; 3. Reagent preparation: reaction system (25 μL), take 15 μL of reaction solution, add 2 μL of template and 8 μL of sterile ultrapure water, mix well and centrifuge for a short time; 4. Reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 63 ℃ for 30s, and the second to third steps are cycled 40 times. 5. Fluorescence channel: FAM;
Growth conditions	37 ℃;18-24h; anaerobic
Storage conditions	-80 ℃
Safety level	1
Separation substrate	The Pigs Gut
application	For rapid detection of Clostridium butyricum
Sharing mode	Public welfare sharing