BeNa Culture Collection
Culture medium | Tryptone: 15.0, soy peptone: 5.0, sodium chloride: 5.0, agar: 15.0,pH:7.3±0.2(25 ℃) |
Subculture procedure | ① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried; |
Growth conditions | 30 ℃;18-24h; aerobic; |
Storage conditions | -80 ℃ |
Safety level | 2 |
morphology | The colony has a diameter of 1-2mm, round, neat edges, opaque, yellow front, raised in the middle, smooth surface, bright surface, moist texture, easy to provoke, G-(red), bacilli, purity: pure |
Sharing mode | Public welfare sharing |