Bacillus subtilis var. niger plate count reference material|BNCC384086 |BNCC

BeNa Culture Collection

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  • Standard substance for the plate count of Bacillus atrophaeus Nakamura-BNCC

Standard substance for the plate count of Bacillus atrophaeus Nakamura

  • Price： Contact
  • number：BNCC384086
  • Packing：Syringa;(9.1±1.5)× 10 ^ 5CFU/mL
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Standard substance for the plate count of Bacillus atrophaeus Nakamura
Culture medium Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure Take out the quality control sample from the refrigerator at -20 ℃, place it at room temperature for balance for 30 min, then open it in the biosafety cabinet, immediately add 5mL of sterile water or sterile physiological saline to redissolve the freeze-dried quality control substance, and prepare the sample suspension after fully mixing, which is the quality control sample with a concentration of 3000 ~ 9000CFU/mL.
Growth conditions 37°C, aerobic, 18-24h;
Storage conditions -20 ℃, dark and refrigerated; ice bag transportation
Safety level 2
Sharing mode Public welfare sharing
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