BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC360992 |
| Description | LB Medium with 20μg/mL Streptomycin |
| Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃) |
| Growth conditions | 37°C; 18-24h; aerobic |
| Subculture procedure | 1. Thaw competent cells on ice (or briefly place in palm or at room temperature until cells are in a slushy state, then quickly return to ice). Add target DNA (plasmid or ligation product) and gently mix by tapping the bottom of the Eppendorf tube. Incubate on ice for 25 minutes. 2. Heat shock at 42°C for 45 seconds, then immediately return to ice and let stand for 2 minutes. Agitation reduces transformation efficiency. 3. Add 700μl antibiotic-free sterile LB medium to the centrifuge tube. Mix thoroughly and incubate at 37°C, 200 rpm for 60 minutes. 4. Centrifuge at 5000 rpm for 1 minute to collect cells. Retain approximately 100 μl supernatant, gently resuspend the pellet by pipetting, and plate onto LB medium containing the appropriate antibiotic. 5. Place plates upside down in a 37°C incubator overnight. For blue-white screening, incubate plates at 37°C for at least 17 hours. |
| Storage conditions | -80°C |
| Sharing mode | Public welfare sharing |
Klebsiella pneumoniae subsp. pneumoniae(Klebsiella pneumoniae subsp. pneumoniae)
BNCC186055
Human adenovirus 17 nucleic acid detection kit (Fluorescent PCR method)(Human adenovirus 17 nucleic acid detection kit (Fluorescent PCR method))
BNCC372790
Lactobacillus crispatus nucleic acid detection kit (Fluorescent PCR method)(Lactobacillus crispatus nucleic acid detection kit (Fluorescent PCR method))
BNCC373716
Human adenovirus 24 nucleic acid detection kit (Fluorescent PCR method)(Human adenovirus 24 nucleic acid detection kit (Fluorescent PCR method))
BNCC375969
Pig origin nucleic acid detection kit (Fluorescent PCR method)(Pig origin nucleic acid detection kit (Fluorescent PCR method))
BNCC378053
Biochemical identification kit for Corynebacterium kutscheri(Biochemical identification kit for Corynebacterium kutscheri)
BNCC378878
- English
- Japanese