BeNa Culture Collection
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| Culture medium | BNCC352243 |
| Description | LB nutrient agar |
| Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, pH:7.0±0.2(25℃) |
| Growth conditions | 37°C; 18-24h; aerobic |
| Subculture procedure | ① Thaw Frozen vials in a water bath (37°C), shaking vigorously to dissolve; ② Disinfect the outer surface of the vial with an alcohol swab, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate the entire thawed solution, and Agar plate onto 1-2 agar plates (approx. 200μL per Agar plate); ④ Spread evenly and incubate under the specified conditions. |
| Storage conditions | -80°C |
| Sharing mode | Public welfare sharing |
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