BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC361029 |
| Description | LB Medium with 50μg/mL Ampicillin |
| Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃) |
| Growth conditions | 37°C; 18-24h; aerobic |
| Subculture procedure | ① Thaw Frozen vial in water bath (37°C), rapidly shake to dissolve; ② Disinfect outer surface of vial with alcohol swab, then transfer to biosafety cabinet for handling; ③ Unscrew cap, aspirate entire thawed solution, and Agar plate onto 1-2 agar plates (approx. 200μL per Agar plate); ④ Spread evenly and incubate under specified conditions. |
| Storage conditions | -80°C |
| Sharing mode | Public welfare sharing |
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