DH5α E. coli (containing pBR322 plasmid)|353847 |BNCC

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Escherichia coli DH5α(pBR322)

Growth conditions	1. Prepare one sterile liquid test tube (containing 5–10 mL of medium) and two 90 mm plates; 2. After disinfecting the vial walls, open the aluminum cap and rubber stopper in a sterile environment. Pipette approximately 0.5 mL of liquid medium into the vial and gently blow to dissolve the bacterial pellet; 3. After complete dissolution, aspirate the entire solution and return it to the liquid test tube for thorough mixing; 4. Aspirate the mixture from the test tube and Agar plate onto agar plates, 200μL per Agar plate; 5. Incubate all test tubes and plates under the specified conditions. Do not seal or wrap plates. Observe after 18–24 hours for visible turbidity in the culture medium and colony or biofilm growth on plates; 6. If no growth is observed, continue incubation until 72 hours. Use as required after successful cultivation.
Subculture procedure	① Prepare one sterile water vial and two 90mm resistant plates;② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture. Break ampoule with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube. After complete dissolution, spread solution onto antibiotic plates (200 μL/Agar plate);④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Pick a single colony and inoculate into 50mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of the bacterial culture as required by the experiment;
Storage conditions	-20°C
Sharing mode	Public welfare sharing