BeNa Culture Collection
info@bncc.com
![Escherichia coli DH5α(pLV[Exp]-EGFP-EF1A>{GI})-BNCC](https://beinabncc.oss-accelerate.aliyuncs.com/News/5d2d278691620d2f2ef113124b587296.jpg "DH5α (containing pLV[Exp]-EGFP-EF1A>{GI} plasmid) E. coli")
| Culture medium | BNCC360877 |
| Description | Min6 Cell-Specific Medium |
| Composition | 89% DMEM-H+1% NEAA+10% FBS |
| Growth conditions | LB + antibiotics, 37°C |
| Subculture procedure | ① Thaw Frozen vials in a water bath (37°C) and rapidly shake to dissolve; ② Disinfect the outer surface of the vial with an alcohol swab, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate the entire thawed solution, and Agar plate onto 1-2 agar plates (approx. 200μL per Agar plate); ④ Spread evenly and incubate under the specified conditions. |
| Storage conditions | -80°C |
| Sharing mode | Public welfare sharing |
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