DH5 α (containing pUC119 plasmid) Escherichia coli|353852 |BNCC

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Escherichia coli DH5α(pUC119)

Subculture procedure	1 Prepare one sterile liquid test tube (containing 5-10mL of culture medium) and two 90mm plates; two After disinfecting the wall of the penicillin bottle, in a sterile environment, open the aluminum cap and rubber stopper in sequence, draw about 0.5mL of liquid culture medium into the bottle, gently blow and dissolve the bacterial slices; three After the bacterial tablets are fully dissolved, aspirate all the dissolved solution and mix it evenly in the liquid test tube; four Draw the mixed solution from the test tube liquid and apply it to the Agar plate at a rate of 200 μ L per Agar plate; five Place all test tubes and plates under the specified conditions for cultivation, and prohibit sealing and wrapping the plates. After 18-24 hours, observe that the culture medium is significantly turbid and that the Agar plate colonies or bacterial moss are growing; six If the strain has not grown, it should be further cultured for 72 hours, and after cultivation, it should be used according to the specific experimental requirements.
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	-80 ℃
Safety level	1
Sharing mode	Public welfare sharing

Escherichia coli DH5α( pUC119)

No.: 353852

Product format: tablets in penicillin bottle

storage conditions: -20 ℃

Biosafety level: 1

Validity: 5 years

usage: pUC119 plasmid extraction

Notes: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened. Keep it at -20℃ for storage if it is not used. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

medium:LB resistant agar medium yeast extract 5.0g, peptone 10.0g,NaCl 10.0g, agar 15.0g, distilled water 1.0 L,pH 7.0. Sterilization at 121 ℃ for 15min. After sterilization, ampicillin sodium is added after cooling down, so that the final content of ampicillin sodium in the culture medium is 50 ug/mL.

Recovery steps:
(1)Prepare a sterile test tube of 5-10ml liquid medium and 2 pieces of 90mm plates;
(2)sterilizing of the penicillin-bottle, under aseptic conditions remove the aluminium cap and rubber plug, draw 0.5ml of liquid culture medium into the vial, aspirate to dissolve the bacterial tablets.
(3)fully dissolved, transfer the solution to the liquid test tube, mix evenly;
(4)inoculate a plate with 0.2ml of the solution, distribute it well in 200ml/plate;
(5)Put all the plates and test tubes under the above culture conditions for cultivation, the plates are not allowed to seal by wrapping. The culture solution is obviously turbid after 18-24 hours, and this indicates the bacterial grows well.
(6) If it does not grow, culture is continued until 72 hours is reached. And the strains can be used when they grow

Growth conditions

Temperature: 37 ℃ Atmosphere: aerobic Time: 18-24h

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