DH5α (containing pET-41a plasmid) E. coli|358947 |BNCC

Essential Information Related Products

Escherichia coli DH5α(pET-41a)

Growth conditions	37°C; 18-24h; aerobic
Subculture procedure	① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL of liquid medium into the lyophilized tube, dissolve completely, then transfer back to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL of bacterial suspension onto a Agar plate and spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions	-80°C
Sharing mode	Public welfare sharing