Stbl3 (containing pACT1:Cas9-GFP, U6:sgTK plasmid) E. coli|359700 |BNCC

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Escherichia coli Stbl3(pACT1:Cas9-GFP, U6:sgTK)

Growth conditions	37°C; 18-24h; aerobic;
Subculture procedure	① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a biosafety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it. Use forceps to crush it; ③ Pipette 0.5mL liquid medium into a lyophilized tube, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once bacterial growth appears.
Storage conditions	-80°C
Sharing mode	Public welfare sharing