DH5 α (containing pRS416 plasmid) Escherichia coli|397125 |BNCC

Essential Information Related Products

Escherichia coli DH5α(pRS416)

Growth conditions	37 ℃; 18-24 hours; aerobic;
Subculture procedure	① Prepare 1 sterile water tube and 2 90mm resistant plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Draw 0.5mL of sterile water and transfer it into a freeze-drying tube. After sufficient dissolution, apply the solution onto resistant plates at a rate of 200 μ L per Agar plate; ④ Place the resistant Agar plate under the specified conditions for cultivation for 18-24 hours and observe it (sealing and wrapping the Agar plate is prohibited); ⑤ Select a single colony and inoculate it into 50mL of liquid LB resistant medium. Incubate overnight at 37 ℃ on a shaker; ⑥ According to experimental requirements, extract an appropriate amount of bacterial solution for plasmid extraction;
Storage conditions	-80 ℃
Sharing mode	Public welfare sharing