BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC361029 |
| Description | LB Medium with 50μg/mL Ampicillin |
| Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃) |
| Growth conditions | 37 ℃; 18-24h; aerobic |
| Subculture procedure | ① Prepare 1 sterile water and 2 90mm resistant tablets; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Draw 0.5mL of sterile water and transfer it into a freeze-drying tube. After sufficient dissolution, apply the solution onto resistant plates at a rate of 200 μ L per Agar plate; ④ Place the resistant Agar plate under the specified conditions for cultivation for 18-24 hours and observe it (sealing and wrapping the Agar plate is prohibited); ⑤ Select a single colony and inoculate it into 50mL of liquid LB resistant medium. Incubate overnight at 37 ℃ on a shaker; ⑥ According to experimental requirements, extract an appropriate amount of bacterial solution for plasmid extraction; |
| Storage conditions | 2-8 ℃ |
| Sharing mode | Public welfare sharing |
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