BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC361023 |
| Description | LB Medium with 50μg/mL Kanamycin |
| Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃) |
| Growth conditions | 37 ℃; 18-24 hours; aerobic; |
| Subculture procedure | ① Dissolve the Frozen vial tube in a water bath (37 ℃) and shake it quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Sharing mode | Public welfare sharing |
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