BeNa Culture Collection
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| Growth conditions | LB + Ampicillin, 37°C (Cloning strain: Stbl3, Promoter: U6, CMV Rep: pUC) |
| Subculture procedure | 1. Dissolution: Upon receiving the plasmid Freeze dried pellet, add 20μl sterile water to the bottom of the tube to dissolve the plasmid. Incubate at Room temperature for 1 minute. 2. Mixing (Plasmid Adsorption): Mix 200μl competent cells + 5–10μl plasmid DNA thoroughly. Incubate on ice for 30 minutes. 3. Heat shock transformation: Incubate at 42°C for 90 seconds; 4. Shrink membrane pores: Ice bath for 2 minutes; 5. Repair culture: Add 800 µl LB liquid medium to each tube; incubate at 37°C for 1 hour at 150 rpm; 6. Screening culture: Spread an appropriate volume (100 µl) of the revived cells onto LB plates with the corresponding antibiotic. Incubate plates upright for 30 min (ensure agar surface is dry), then invert and incubate for 12–16 hours until colonies appear. 7. Extraction: Transfer a single colony to LB liquid medium with the corresponding antibiotic, shake-incubate for 12-16 hours, and extract the plasmid as required for the experiment. |
| Storage conditions | 2-8°C |
| Sharing mode | Public welfare sharing |
Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC396427
Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC396428
Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC398205
Human rhinovirus 31 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 31 nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC398206
Plasmid DNA of White Spot Syndrome Virus (WSSV)(Plasmid DNA of White Spot Syndrome Virus (WSSV))
BNCC397425
Plasmid DNA of Necrotizing Hepatopancreatitis (NHP)(Plasmid DNA of Necrotizing Hepatopancreatitis (NHP))
BNCC397429
Lactobacillus intestinalis DNA standard
BNCC397806
Pseudomonas aeruginosa Migula nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC397776
Mycoplasma bovis DNA standard
BNCC397624
Acinetobacter baumannii nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC397541
Aspergillus fumigatus nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC397502
Human rhinovirus 61 RNA lab quality control
BNCC397268
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