pACYC-MS2-TBEV-3'UTR|391565 |BNCC

BeNa Culture Collection

BNCC > Plasmid > pACYC-MS2-TBEV-3'UTR
  • pACYC-MS2-TBEV-3'UTR-BNCC

pACYC-MS2-TBEV-3'UTR

  • Price： $ 112
  • number：BNCC391565
  • Packing：Freeze dried pellet
Add to cart
View Cart
COA
Essential Information Related Products
pACYC-MS2-TBEV-3'UTR
Growth conditions 30°C; 18-24h; aerobic
Subculture procedure ①Dissolution: Add 20μL sterile water to lyophilized plasmid powder for dissolution, incubate at Room temperature for 1 min;② Mixing: Combine 200μl competent cells with 5-10μl plasmid DNA, mix thoroughly, and incubate on ice for 30 min; ③ Heat shock transformation: Incubate at 42°C for 90 s; ④ Shrink membrane pores: Ice bath for 2 min;⑤ Repair culture: Add 800 μl liquid medium to each tube; incubate on a 37°C shaker for 1 hour (150 rpm);⑥ Screening Culture: Spread an appropriate volume (100 μl) of the revived cells onto LB plates with the corresponding antibiotic. Incubate plates upright for 30 minutes (ensure agar surface is completely dry), then invert and incubate for 12-16 hours until colonies appear.⑦ Extraction: Transfer a single colony to the corresponding antibiotic-supplemented LB liquid medium. Incubate with shaking for 12–16 hours. Extract the plasmid as required for the experiment.
Storage conditions 2-8°C
Sharing mode Public welfare sharing
Related Products
Popular Products
Please set your password: