BeNa Culture Collection
info@bncc.com
| Growth conditions | 37℃； 18-24h; aerobic |
| Subculture procedure | ① Dissolve: Add 20 μ L of sterile water to the plasmid powder and let it stand at Room temperature for 1 minute; ② Mixing: Mix 200 μ l of competent cells with 5-10 μ l of plasmid DNA and place on ice for 30 minutes; ③ Heat shock introduction: let stand at 42 ℃ for 90 seconds; ④ Shrinkage film pores: ice bath for 2 minutes; ⑤ Repair culture: Add 800 μ l of liquid culture medium to each tube and culture on a shaker at 37 ℃ for 1 hour (150 r/min); ⑥ Screening and cultivation: Apply an appropriate volume (100 μ l) of revived cells onto corresponding resistant LB plates, place them upright in a petri dish for 30 minutes (the agar surface must be dry), invert and culture for 12-16 hours, and colonies will appear; ⑦ Extraction: Select monoclonal colonies into the corresponding resistant LB liquid medium, shake and culture for 12-16 hours, and extract plasmids according to experimental needs. |
| Storage conditions | 2-8 ℃ |
| Sharing mode | Public welfare sharing |
Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC396427
Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC396428
Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC398205
Human rhinovirus 31 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 31 nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC398206
Plasmid DNA of White Spot Syndrome Virus (WSSV)(Plasmid DNA of White Spot Syndrome Virus (WSSV))
BNCC397425
Plasmid DNA of Necrotizing Hepatopancreatitis (NHP)(Plasmid DNA of Necrotizing Hepatopancreatitis (NHP))
BNCC397429
Feline leukemia virus nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC397971
Genomic DNA from Helicobacter pylori
BNCC397895
Lactobacillus intestinalis DNA lab quality control
BNCC397809
Mycoplasma fermentans DNA standard
BNCC397635
Mycoplasma fermentans DNA lab quality control
BNCC397631
Primers for the detection of Salmonella enterica subsp. enterica serovar Montevideo
BNCC397331
- English
- Japanese