BeNa Culture Collection
info@bncc.com
| Growth conditions | 37°C; 18–24 h; aerobic |
| Subculture procedure | ① Dissolution: Add 20 μL of sterile water to the plasmid Freeze dried pellet to dissolve it, and let stand at Room temperature for 1 min; ② Mixing: Mix 200 μL of competent cells with 5–10 μL of plasmid DNA, and incubate on ice for 30 min; ③ Heat shock transformation: Incubate at 42°C for 90 s; ④ Shrink-wrap: Incubate on ice for 2 min; ⑤ Repair culture: Add 800 μL of liquid medium to each tube and incubate on a shaking incubator at 37 °C for 1 h (150 r/min); ⑥ Screening: Spread an appropriate volume (100 μl) of resuspended cells onto the corresponding resistance LB agar plate; incubate the Agar plate upright for 30 min (ensuring the agar surface is completely dry), then incubate inverted for 12–16 h until colonies appear; ⑦ Isolation: Transfer a single colony to the corresponding antibiotic-containing LB liquid medium, incubate with shaking for 12–16 h, and extract the plasmid as required by the experiment. |
| Storage conditions | 2–8°C |
| Sharing mode | Public welfare sharing |
Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC396427
Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC396428
Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC398205
Human rhinovirus 31 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 31 nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC398206
Plasmid DNA of White Spot Syndrome Virus (WSSV)(Plasmid DNA of White Spot Syndrome Virus (WSSV))
BNCC397425
Plasmid DNA of Necrotizing Hepatopancreatitis (NHP)(Plasmid DNA of Necrotizing Hepatopancreatitis (NHP))
BNCC397429
Pseudomonas aeruginosa Migula nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC397776
Mycoplasma bovis DNA lab quality control
BNCC397620
Acinetobacter lwoffii nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC397508
Streptococcus constellatus subsp. constellatus nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC397503
Human rhinovirus 61 RNA lab quality control
BNCC397268
Human rhinovirus 61 RNA lab quality control
BNCC397267
- English
- Japanese