BeNa Culture Collection
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| Growth conditions | 37°C; 18–24 hours; aerobic; |
| Subculture procedure | ① Dissolution: Add 20 μL of sterile water to the plasmid Freeze dried pellet to dissolve it, and let stand at Room temperature for 1 minute; ② Mixing: Mix 200 μL of competent cells with 5–10 μL of plasmid DNA, and incubate on ice for 30 minutes; ③ Heat shock transformation: Incubate at 42°C for 90 seconds; ④ Shrink wrap: Incubate on ice for 2 min; ⑤ Recovery culture: Add 800 μL of liquid medium to each tube and incubate on a shaking incubator at 37 °C for 1 h (150 r/min); ⑥ Screening: Spread an appropriate volume (100 μl) of resuspended cells onto the corresponding resistance LB agar plate; incubate the Agar plate upright for 30 min (ensuring the agar surface is completely dry), then incubate inverted for 12–16 h until colonies appear; ⑦ Isolation: Transfer a single colony to the corresponding antibiotic-containing LB liquid medium, shake for 12–16 h, and extract the plasmid as required by the experiment. |
| Storage conditions | 2–8°C |
| Sharing mode | Public welfare sharing |
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Plasmid DNA of Peach Leaf Curl Virus (TSV)(Plasmid DNA of Peach Leaf Curl Virus (TSV))
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Plasmid DNA of Prawn yellow head virus(Plasmid DNA of Prawn yellow head virus)
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Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
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Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
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Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC398205
Human rhinovirus 36 nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC397972
Lactobacillus intestinalis DNA lab quality control
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Mycoplasma fermentans DNA standard
BNCC397634
Mycoplasma bovis DNA standard
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Negative Control Inoculated Carrier (slide)
BNCC397524
Genomic DNA from Roseburia faecis
BNCC397148
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