BeNa Culture Collection
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| Growth conditions | 37°C; 18–24 hours; aerobic; |
| Subculture procedure | ① Dissolution: Add 20 μL of sterile water to the plasmid Freeze dried pellet to dissolve it, and let stand at Room temperature for 1 min; ② Mixing: Mix 200 μL of competent cells with 5–10 μL of plasmid DNA, and incubate on ice for 30 min; ③ Heat shock transformation: Incubate at 42°C for 90 s; ④ Shrink wrap: Incubate on ice for 2 min; ⑤ Repair culture: Add 800 μL of liquid medium to each tube and incubate on a shaking incubator at 37°C for 1 h (150 r/min); ⑥ Screening culture: Spread an appropriate volume (100 μl) of resuspended cells onto the corresponding resistance LB agar plate; incubate the Agar plate upright for 30 min (ensuring the agar surface is completely dry), then incubate inverted for 12–16 h until colonies appear; ⑦ Isolation: Transfer single colonies to the corresponding antibiotic-containing LB liquid medium, incubate with shaking for 12–16 h, and extract plasmids as required for the experiment. |
| Storage conditions | 2–8°C |
| Sharing mode | Public welfare sharing |
Plasmid DNA of Infectious Hypodermal and Hematopoietic Necrosis Virus(Plasmid DNA of Infectious Hypodermal and Hematopoietic Necrosis Virus)
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Plasmid DNA of Peach Leaf Curl Virus (TSV)(Plasmid DNA of Peach Leaf Curl Virus (TSV))
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Plasmid DNA of Prawn yellow head virus(Plasmid DNA of Prawn yellow head virus)
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Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
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Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive)(Rotavirus A nucleic acid reference (Heat inactivated) (Strongly positive))
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Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive)(Human rhinovirus 53 nucleic acid reference (Heat inactivated) (Strongly positive))
BNCC398205
Mycoplasma fermentans DNA standard
BNCC397635
Mycoplasma bovis acid reference (Heat inactivated) (Strongly positive)
BNCC397627
Candida tropicalis nucleic acid reference (Heat inactivated) (Strongly positive)
BNCC397517
Lactobacillus intestinalis DNA standard
BNCC397453
Quantitative strains of clostridium sporogenes
BNCC397431
Human rhinovirus 61 RNA standard
BNCC397271
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