pCDNA3.1-ERBB2(human)-9-3×Linker-EGFP|363942 |BNCC

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pCDNA3.1-ERBB2(human)-9-3×Linker-EGFP

Subculture procedure	① Dissolution: Add 20 μL sterile water to the plasmid powder for dissolution, let stand at Room temperature for 1 min; ② Mix: Combine 200 μl competent cells with 5-10 μl plasmid DNA, mix thoroughly, and incubate on ice for 30 min; ③ Heat shock transformation: Incubate at 42°C for 90 s; ④ Shrink membrane pores: Ice bath for 2 min; ⑤ Repair culture: Add 800 μl liquid medium to each tube, incubate on a 37°C shaker for 1 h (150 rpm); ⑥ Screening culture: Spread an appropriate volume (100 μl) of thawed cells onto LB plates with the corresponding antibiotic. Incubate plates upright for 30 min (ensure agar surface is completely dry), then invert and incubate for 12-16 hours until colonies appear; ⑦ Extraction: Pick a single colony into the corresponding antibiotic-containing LB liquid medium. Incubate with shaking for 12–16 hours. Extract the plasmid as required for the experiment.
Growth conditions	LB + antibiotics, 37°C
Storage conditions	2-8°C
Safety level	1
Sharing mode	Public welfare sharing