BeNa Culture Collection
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| Culture medium | Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use. |
| Subculture procedure | When this standard is used, it is moved to room temperature to be completely dissolved, shaken evenly, and used after instantaneous centrifugation to avoid repeated freezing and thawing as much as possible. |
| Growth conditions | 37 ℃;48-72h;5% CO2 |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 0.5-1mm, round, with neat edges, translucent, gray-white front, convex middle, bright surface, smooth surface, moist texture, easy to pick up, G-(red), Vibrio |
| Separation substrate | Gastritis patient stomach |
| application | Helicobacter pylori, also known as Helicobacter pylori, its DNA standard is mainly used for positive control, nucleic acid detection kit development, and related research. |
| Sharing mode | Public welfare sharing |
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