BeNa Culture Collection
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| Culture medium | Columbia blood plate (English name: CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use. |
| Subculture procedure | When this standard is used, it is moved to room temperature (20~25 ℃) for re-melting, oscillates evenly, centrifuges briefly, and is temporarily stored for use in a low temperature environment on ice to avoid repeated freezing and thawing. |
| Growth conditions | 37 ℃;2-3 days; Aerobic gas environment; |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| application | It is suitable for qualitative and quantitative research and analysis of Bordetella pertussis, verification and evaluation of measurement methods and laboratory quality control. |
| Sharing mode | Public welfare sharing |
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