Quantitative strain of Staphylococcus epidermidis|BNCC361916 |BNCC

Essential Information Related Products

Staphylococcus epidermidis

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	(1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions	37 ℃;18-24 hours; Aerobic;
Storage conditions	-80 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, light color, convex in the middle, smooth and bright surface, moist texture, easy to stir up, no pigment, G + (blue-purple), cocci, purity: pure
Sharing mode	Public welfare sharing