Candida albicans quantitative strain|BNCC358452 |BNCC

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Candida albicans

Culture medium	YM medium (YM): yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions	28 ℃;24-48h; Aerobic;
Growth characteristics	Cells round or oval, polygonal budding.
Storage conditions	-20 ℃
morphology	milky white, raised, moist, viscous, G + cocci
application	Research; Analytical testing. "Technical Specifications for Disinfection" Bacteria for Testing Sterilization Effect of Traditional Chinese Medicine Therapeutics, "GB 4789.15-2010 Mold and Yeast Count" Positive Control Strain, 2015 Edition Chinese Pharmacopoeia Quality Control Strain.
Sharing mode	Public welfare sharing

Candida albicans

Strain number:BNCC 358452 / CMCC(F)98001

Name: Candida albicans

Product format: quantitative bacteria tablets Number of viable bacteria: (1.2±0.2)×10^6CFU/bottle

Product batch: 220323 Valid until: 2024.03.22

Inspection date: 2022.03.24 Sampling ratio: ≥2%

Report number: RS22358452-1 Biosafety level: 1, handle in ultra-clear table or safety cabinet

Item	description	results
stain microscopy	gram negative and positive, cell morphology observed under microscope	G + cocci
colony morphology	observation of single colony morphology	milky white, raised, moist, sticky
Purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	(1.2±0.2)×10^6CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	qualified

Product list: One bottle of (1.2±0.2)×10^6CFU quantitative strain, one bottle of 1mL dissolution solution and one copy of strain certificate;

Validity period: unopened, below -20 ℃, 24 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. after full dissolution, it is (1.2±0.2)×10^6CFU/bottle, according to the test requirements according to the proportion of suction can be;

4. Please use up immediately after opening, and do not store it;

Notes:

① this product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

(2) used bacteria-carrying consumables, please autoclave and discard them.

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