Quantitative strain of Pseudomonas aeruginosa|BNCC354700 |BNCC

Essential Information Certificate Related Products

Pseudomonas aeruginosa

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Growth conditions	37 ℃; Aerobic
Growth characteristics	The strain was cultured at 37 ℃ for 3 days on nutrient gravy agar medium. The bacteria were rod-shaped, 0.61 μm × 1.22~2.13 μm, Gram-negative and did not produce spores.
Storage conditions	-80 ℃
Separation substrate	Blood culture
application	Research; Analytical testing. Quality control strains of chinese pharmacopoeia, 2015 edition.
Sharing mode	Public welfare sharing

Pseudomonas aeruginosa

Strain number: BNCC 354700 / CMCC10104

Name: Pseudomonas aeruginosa

Product format: quantitative bacteria tablets Number of viable bacteria:0.5- 1.0x10⁵CFU/bottle

Product batch: 220322 Valid until: 2024.03.21

Inspection date: 2022.03.23 Sampling ratio: ≥2%

Report number: RS22354967-1 Biosafety level: 1, handle in ultra-clear table or safety cabinet

Item	Inspection description	results
stain microscopy	gram negative and positive, cell morphology observed under microscope	G-bacilli
colony morphology	observation of single colony morphology	light yellow, smooth, moist, raised
Purity	non-selective medium culture, colony observation and microscopic examination	No contaminated bacterial
Reference value of live bacteria	Non-selective medium plate, viable count	72917CFU/bottle
Phenotypic and Genotypic Analysis	phenotypic testing, gene sequencing	qualified
test conclusion	After the BNCC quality control inspection system quality inspection, the product is qualified

Product list: 0.5- 1.0x10⁵CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1;

Validity period: unopened, below -20 ℃, 12 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. After fully dissolved, it is 0.5- 1.0x10⁵CFU/bottle, which can be sucked in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

② Used bacteria-carrying consumables, please autoclave and discard them.

Download certificate