BeNa Culture Collection
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| Culture medium | BNCC372404 |
| Description | Reinforced Clostridial Medium (RCM) |
| Composition | Yeast Extract:3.0g, Beef Infusion Powder:10.0g, Glucose:5.0g, Pancreatic Digest of Casein:10.0g, NaCl:5.0g, Soluble Starch:1.0g, L-Cysteine Hydrochloride(C3H7NO2S.HCl):0.5g, CH3COONa:3.0g, Agar:0.5g, pH:6.8±0.1(25℃) |
| Growth conditions | 37 ℃; 24-48 hours; anaerobic |
| Subculture procedure | ① Prepare 1-2 plates and sterile water (deoxygenated in an anaerobic environment for 24 hours in advance); ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, ignite the top with an alcohol burner, quickly drop sterile water on it to make it break, and then use tweezers to crush it; ③ Pipette 0.5mL of sterile water into the lyophilized tube, fully dissolve the bacterial powder, and then dispense 200μL per Agar plate, spreading evenly; ④ Place the plates under the above-mentioned culture conditions and use them once the bacteria grow. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 1-2mm, circular, with irregular edges, opaque, light yellow on the front, flat in shape, rough on the surface, dry in texture, difficult to stir, non-pigment-producing, G+ (blue-purple), bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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