Clostridium difficile 1351|344673 |BNCC

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Clostridium difficile (Hall and O'Toole) Prevot

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 48-72h; anaerobic;
Subculture procedure	①Prepare 1-2 of the above plates (placed in an anaerobic environment for 24 hours of deoxygenation in advance) with typical bacterial colonies; ② After disinfecting the surface of the tablet, open it in the safety cabinet; ③ Pick 1-2 rings of agar colonies using sterile inoculation rings and transfer them to new culture medium; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony has a diameter of 1-2mm, irregular shape, wavy edges, opaque, white front, raised middle, smooth surface, bright surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing