Top 10 cloned strains of Escherichia coli|353819 |BNCC

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Escherichia coli Top10

Culture medium	BNCC360873
Description	LB Medium with 50μg/mL Streptomycin
Composition	Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions	37 ℃; 24-48 hours; aerobic;
Subculture procedure	① Dissolve the Frozen vial in a water bath (37 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing