Botrytis cinerea|354192 |BNCC

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Metarhizium kusanagiense (Kobayasi & Shimizu) Kepler et al.

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	28 ℃; 15 days; aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi, with obvious white colonies and sparse and protruding hyphae on the comprehensive PDA medium, and a light yellow color on the back of the medium
Sharing mode	Public welfare sharing

Metarhizium kusanagiense

Storage conditions: 2~8 ℃

No. 354192

Product format: agar slant in 14mm test tube

Validity period : growing culture, in 30 dyas

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, integrated PDA,15 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:
(1) Prepare 1-2 pieces of PDA plates;
(2) Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;
(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;
(4) Lay flat the small pieces to the center of the agar plate;
(5) Put the plates under the above culture conditions, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability， in 15days strain layer is obvious
colony morphology:	small filamentous fungi, with obvious colonies, white, sparse and convex hyphae, and light yellow on the back of the medium on the integrated PDA medium.
Conclusion:	good viability, no abnormal colony morphology, qualified ;

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