Thermophilic and Lipophilic Bacillus subtilis|354213 |BNCC

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Geobacillus stearothermophilus (Donk) Nazina et al.

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	55 ℃; 18-24h; aerobic
Subculture procedure	① Prepare 1 sterile water and 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube to dissolve and mix thoroughly; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with irregular edges, opaque, yellow on the front, light in color, raised in the middle, dull on the surface, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing