BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 3-4 days; anaerobic; |
| Subculture procedure | ① Prepare 1-2 pieces of the aforementioned agar plates (pre-incubated in an anaerobic environment for 24 hours to remove oxygen); ② Open them in a safety cabinet, ignite the top with an alcohol burner, quickly drop sterile water to rupture them, and then use tweezers to break them into pieces; ③ Pipette 0.5mL of sterile water into the lyophilized tubes, fully dissolve the bacterial powder, and then dispense 200μL per Agar plate, spreading it evenly; ④ Incubate the agar plates under the aforementioned culture conditions until the bacterial strain grows, and then they are ready for use. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colony is 1-2mm in diameter, circular, with neat edges, translucent, raised in the middle, smooth on the surface, moist in texture, and does not produce pigments. It is Gram-negative (red), a bacillus, and pure |
| Sharing mode | Public welfare sharing |
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