Bacillus subtilis subspecies|361868 |BNCC

BeNa Culture Collection

Bacillus subtilis subspecies-BNCC
  • BNCC
  • Bacillus subtilis subsp. subtilis-BNCC

Bacillus subtilis subsp. subtilis

  • Price: $ 186
  • number:BNCC361868
  • MPs:
    The colony diameter is 2-4mm, circular, with irregular edges, opaque, gray white on the front, light colored, flat in shape, smooth on the surface, dark on the surface, sticky in texture, easy to pick up, does not produce pigments, G+(blue purple), Bacillus, purity: pure
Essential Information Related Products
Bacillus subtilis subsp. subtilis
Culture medium BNCC352239
Description Nutrient Agar
Composition Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions 30 ℃; 24-48 hours; aerobic
Subculture procedure ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions 2-8 ℃
Safety level 1
morphology The colony diameter is 2-4mm, circular, with irregular edges, opaque, gray white on the front, light colored, flat in shape, smooth on the surface, dark on the surface, sticky in texture, easy to pick up, does not produce pigments, G+(blue purple), Bacillus, purity: pure
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